Label-free detection of bacterial RNA using polydiacetylene-based biochip.
نویسندگان
چکیده
We developed a simple and effective polydiacetylene-based, label-free multiplex DNA chip for the detection of various pathogenic microorganisms. A novel immobilization method of PDA vesicles on glass slides was exploited using α-cyclodextrin (α-CD). The surface topography of the efficiently immobilized PDA vesicles was confirmed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Then, oligonucleotides complementary to rRNAs of three pathogenic bacteria were conjugated to the PDA vesicles. Finally, crude lysate of pathogenic bacteria was applied to the PDA biochip. The pathogenic bacteria were specifically detected by DNA-RNA hybridization in an hour. The new PDA sensor was effective in detecting multiple pathogenic bacteria easily and accurately without rigorous purification, amplification, and labeling of their genetic components.
منابع مشابه
Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes
Development of a miniaturized biosensor system that can be used for rapid detection and counting of microorganisms in food or water samples is described. The developed microsystem employs a highly sensitive impedimetric array of biosensors to monitor the growth of bacterial colonies that are dispersed across an agar growth medium. To use the system, a sample containing the bacteria is cultured ...
متن کاملLabel-free detection with polydiacetylene vesicle immobilized on the substrate
Polyacetylene vesicle has attracted attentions due to its unique chromatic properties, such as blue-to-red transition. The liposome-like vesicle was immobilized on solid substrates to detect small quantity analytes. In this case, fluorescence was used for detection. We successfully detected protein and bacteria using antibodies or aptamers on the vesicles as probes. Recently, we developed a dif...
متن کاملMultiplex 16S rRNA‐derived geno‐biochip for detection of 16 bacterial pathogens from contaminated foods
Foodborne diseases caused by various pathogenic bacteria occur worldwide. To prevent foodborne diseases and minimize their impacts, it is important to inspect contaminated foods and specifically detect many types of pathogenic bacteria. Several DNA oligonucleotide biochips based on 16S rRNA have been investigated to detect bacteria; however, a mode of detection that can be used to detect divers...
متن کاملRéalisation d'une plateforme biopuce sans marquage basée sur la lithographie douce
Enhancement of biochips and biochips scanners is a crucial issue in the actual industry that needs more sensitive and less expensive molecular analysis tools. In this aim, several points have to be re-examined: detector sensitivity, chip density, labeling. In this perspective, we have developed several different techniques based on soft lithography to pattern molecules. We also have developed a...
متن کاملLabel-free electrochemical biosensors for food and drug application
In food sector, there is a huge demand for rapid, reliable, user & eco-friendly biosensors to analyse the quality and safety of food products. Biosensor based methodology depends upon the recognition of a specific antigens or receptors by corresponding antibodies, aptamers or high-affinity ligands. The first scientifically commercialised sensors were the electrochemical sensors used for the ana...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Biosensors & bioelectronics
دوره 35 1 شماره
صفحات -
تاریخ انتشار 2012